Protein Changes and Antioxidant Enzymes Activity on Barley Inbred Lines Under Water Shortage

Document Type : Research Paper

Authors

1 Assistant professor, Agricultural Biotechnology Department, Imam Khomeini International University, Qazvin

2 MSc. Student of Agricultural Biotechnology, Imam Khomeini International University, Qazvin

Abstract

So far, extensive research projects have been sort out to study effects of drought stress on the plant antioxidant enzymes activity, while there is some information lacking of such studies on the field condition. In the present research project, total protein content and enzymes activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POX) were analyzed under water shortage stress using spectrophotometer and gel assay procedures on a randomized factorial design for five inbred lines of barley. Amount of total protein was significantly increased under water shortage, whether amount of chlorophyll decreased. Activity of SOD and CAT, POX were significantly increased under water shortage stress. Level of enhancement in the activity of POX was more intensive for watering plants than drought stress plants. Also increased activities of POX and SOD were significant under drought stress in comparison to rain fall condition. The kinetic assays were confirmed by isozymic analysis of gel assay. Results indicated that among all studied enzymes, activity of APX was low and didn't have important role in plant protection under water stress, whether POX might have a key role as important enzyme to increase tolerance to drought stress in Hordeum vulgare inbred lines. Also, such activity of enzymes is suggested that different isozymes might be induced and exhibit activity depends on the water shortage stress level in barley.

Keywords

Volume 1, Issue 2 - Serial Number 2
February 2010
Pages 1-10

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