In vitro Belblet Formation of Mooseer (Allium hirtifolium)

Document Type : Research Paper

Authors

1 M.Sc. Student of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

2 Assistant Professor of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

3 Associate Professor of biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

Abstract

In order to study bulblet formation of mooseer from stem disc explants two separate experiments were conducted. In the first experiment, the effect of two concentrations (0.5 and 1 mg/L) of naphthaleneacetic acid (NAA) and deferent levels (0.5, 1, 2 and 3 mg/L) of benzyladenine (BA) and in the second experiment, the effect of sugar source (sucrose and common sugar) and sugar concentration (30, 45, 60 and 90 g/L) were tested. All of explants were culture in MS medium. Results showed that in media without BA no bulblet were generated and just callus were produced. The highest numbers of bulblets (4.65) were observed in 2 mg/L BA and 1 mg/L NAA which had no significant difference with 1 mg/L BA and 0.5 mg/L NAA (4.55). The biggest bulblets were produced in 3 mg/L BA and 0.5 mg/L NAA which had no significant difference with 3 mg/L BA and 1 mg/L NAA. The standard (MS medium without hormones) had the least number of bulblets. In second experiment the effect of sugar source and combination effect of sugar source and sugar concentrations were not significant on bulblet production. This showed that common sugar can be use instead of sucrose. By increasing sugar concentration up to 60 g/L bulblet production increased. The lowest number of bulblet was observed in 30 and 90 g/L sugar. In highest concentration of sucrose bigger bulblets were produced compare with other treatments.

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Volume 1, Issue 2 - Serial Number 2
February 2010
Pages 65-73

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