Investigation of Protein Diversity of Olive (Olea europaea L.) Genotypes Through Electrophoresis of Seed Storage Proteins

Document Type : Research Article

Authors

1 M.Sc. Student, Department of Biotechnology, Faculty of Agriculture, University of Shahed, Tehran, Iran

2 Associate Professor, Department of Horticultural sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

3 Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Abstract

Botanical hereditary reserves have special importance in racial direction of plants and preserving them is very important in national and international viewpoint. Recognizing and introducing suitable species for different climates are among essential needs to develop olive cultivation in Iran. There are various olive genotypes in Iran that recognizing and introducing such variety can accelerate genetic basis development of these species. The current study has been performed in order to inspect protein diversity of 23 olive genotypes from the viewpoint of electrophoresis patterns of seed storage proteins. The electrophoresis results of extracted proteins revealed the existence of 15 strips according to proportional movement on gel that three poly-peptide groups have been noticed which had 6 repeatable strips of {P1 (21 KDa), P2 (25 KDa), P3 (28 KDa), P4 (31 KDa), P5 (35 KDa), P6 (45 KDa)} was observed in all genotypes. There were only 9 polymorphism strips among genotypes. The considerable diversity was seen in pattern of 9 bands of protein which were studied. Cluster analysis profile of proteins regrouped them into three clusters included one, two and twenty genotypes in each cluster.

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References

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Volume 8, Issue 2
May 2017
Pages 215-221

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