Evaluation of Genetic Diversity of Asparagus (Asparagus officinalis L.) Accessions from Mazandaran Province Using Morphological Traits and RAPD Molecular Marker

Document Type : Research Article

Authors

1 Associate Professor, Department of Horticultural Sciences, College of Agricultural and Natural Resources, University of Tehran, Karaj

2 MSc Graduated, Department of Horticultural Sciences, College of Agricultural and Natural Resources, University of Tehran, Karaj

3 Research Assistant Professor, Department of Natural Resources, Mazandaran Agricultural and Natural Resources Research Center, (AREEO), Sari, Iran

Abstract

In this study genetic diversity of 48 asparagus accessions from Mazandaran province was evaluated using 24 morphological characteristics and 35 accessions using Random Amplified Polymorphism DNA (RAPD) molecular marker. Based on factor analysis, nine main factors explained 80 percentage of total variation and spear characters were more effective in accessions classification. Based on cluster analysis of studied morphological traits accessions divided into two main groups. Seventeen out of 90 primers could produced sharp and evaluable bands and the amount of polymorphism was 98.11%. Based on cluster analysis of RAPD analysis using unweighed pair group method with artithemtical average (UPGMA) method, accessions were classified into seven groups at 0.42 of similarity coefficient and asparagus accessions from Mazandaran were separated from Mary Washington UC350 cultivar, A. persicus and Taleghan accession. In this research, using morphological traits and RAPD marker, Mary Washington UC350 cultivar was separated from asparagus accessions from Mazandaran.

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References

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Volume 9, Issue 1
May 2017
Pages 179-193

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