Effect of Carbon Nanotubes on In Vitro Germination of Seeds of Two Strawberry Cultivars

Document Type : Research Article

Authors

1 MSc Graduated, Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz

3 Professor, Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz

4 Assistant Professor, Zanjan Agricultural and Natural Resources Research and Education Center, Zanjan, Iran

Abstract

Strawberry seed germination is long and non-uniform and variable, which can cause problems for the plant breeding programs. Reducing the time of germination for strawberry seeds and achieving a high percentage of germination, will allow breeders to easily assess the strawberry germ plasm. Recently, carbon nanotubes have been used to improve seeds germination of plants. Therefore, this experiment was conducted to evaluate the effect of carbon nanotubes on in vitro germination of strawberry seeds. The test was carried out through a factorial experiment based on a completely randomized design with 5 replicates. Factors included two in vitro culture media (B5 and MS), different concentrations of carbon nanotubes (0 (control), 5, 10, 20 and 40 µg/ml) and two cultivars of strawberries (Paros and Camarosa). At the end of experiment (40 days) germination indexes including germination percentage, germination rate, time to 50% of final germination, means of radicle and plumule length (two weeks after germination) were evaluated. The results showed that seeds of the two cultivars cultured on MS and B5 media containing 10, 20 and 40 µg/ml nanotube had no sign of germination. In 0 (control) and 5 µg/ml, seeds on both culture media germinated significantly compared to the other treatments. In most indexes, control treatment was better and significantly different among treatments. The results showed that carbon nanotubes in high concentrations restricted the strawberry seed germination possibly because of toxicity induction for the seed embryo.

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References

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Volume 9, Issue 1
May 2017
Pages 79-89

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