Response of Tissue Culture Derived Potato Plantlets to Nitrogen and Jasmonic Acid under Hydroponic Condition

Document Type : Research Paper

Authors

1 M.Sc, In Crop Production and Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

2 Associate Professor of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

3 Assistant professor of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

4 Assistant professor of Crop Production and Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

Abstract

The response of potato plantles derived from tissue culture to nitrogen and jasmonic acid adding under hydroponic condition was studied in a factorial experiment based on a complete randomized design. The study was carried out in a research green house.  Experimental treatments included 3 levels of nitrogen (0, 50 and 100 mg/l of culture media) from the source of ammonium nitrate and 4 levels of jasmonic acid (0, 1, 5 and 10 µM) in three replicates. Total number and weigh of minitubers; average weight of minituber; plant height and number of leaves; and harvest index were studied. The results showed that the highest total number and weight of minituber, average weight of minituber, and harvest index obtained from the treatment of 50 mg nitrogen plus 5 µM jasmonic acid. Also the highest plant stem and leaf number obtained from the treatments of 50 mg nitrogen without jasmonic acid and 100 mg nitrogen plus 1 µM jasmonic acid respectively. The lowest number and total weight of minituber and leaf number; belonged to the treatment of 1 µ M jasmonic acid without nitrogen.

Keywords