The Effect of Various Factors on Browning of Date Palm (Phoenix dactylifera L.) Explant In Vitro

Document Type : Research Article

Authors

1 MSc Graduated Student, Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Academic Member of the Date Palm and Tropical Fruits Research Institute, Ahvaz, Iran

Abstract

Explant browning is the main problem in date palm tissue culture. This study was conducted to investigate the effect of various factors on browning of date palm explants, by five independent experiment including surface sterilization of explants with sodium hypochlorite (one-step vs. two-step), controlling explants browning through putrescine application (0, 4 and 8mM), medium strength (full, 1/2, 1/4MS), Zn source and concentration (MS without Zn, MS + 2.43 and 4.86 mg/l bulk Zn, MS+ 2.43, 4.86 and 9.72 mg/l ZnO nanoparticles) and culture systems (Temporary Immersion Bioreactor, vessel with filter on lid, and sealed vessel). Results showed that the best method for explants sterilization was one-step sterilization for 20min. with vigorous shaking. For browning reduction, the best results were obtained with 4mM putrescine, full MS strength, MS + 4.86 mg/l bulk Zn and culture in vessels with filters on lids.

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References

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Volume 10, Issue 1
June 2018
Pages 33-45

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