Effect of Multi-walled Carbon Nanotubes and Salinity Stress on Morphological and Phytochemical Characteristics of Satureja rechingeri Jamzad In Vitro

Document Type : Research Article

Authors

1 MSc Graduated Student, Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran

2 Associate Professor, Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran

3 Assistant Professor, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Tehran, Iran

Abstract

In order to evaluate the effect of multi-walled carbon nanotubes and salinity stress on morphological and phytochemical characteristics of Satureja rechingeri, a factorial experiment was conducted in vitro based on completely randomized design (CRD) with four replications. The first factor was the concentrations of 0, 50, 100 and 200μg/ml of carbon nanotubes and the second factor was the concentrations of 0, 50, 100 and 200mM NaCl. The number and length of shoots and roots, fresh and dry weight of the aerial parts, caffeic acid and rosmarinic acid content were evaluated. By increasing of NaCl concentration from 0 to 200mM, significant reduction in shoots fresh weight, root and shoot length and the number of shoots and roots was observed. Among different concentrations of carbon nanotubes, 100 μg/ml caused a significant increase in the aerial parts dry weight. Under salinity stress, carbon nanotubes improved the number and length of roots and the number and dry weight of shoots. By increasing of carbon nanotubes, caffeic acid and rosmarinic acid content were increased, while caffeic acid and rosmarinic acid content was significantly decreased under different NaCl concentrations. This finding shows carbon nanotubes alleviate the stress condition.  

Keywords

Main Subjects

References

آریان، ز. 1392. بررسی تأثیر تنش شوری بر رشد و نمو، عملکرد، مواد مؤثره و پارامترهای فیزیولوژیکی دو گونه مرزه Satureja rechingeri و Satureja khuzistanica. پایان‌نامه کارشناسی‌ارشد، دانشگاه تهران، 154 صفحه.
Al-Amira, H. and Cracker, L. E. 2007‏. In vitro selection for stress tolerant spearmint. Issues in New Crops and New Uses, 306-310.
Ali, R. M. and Abbas, H. M. 2003. Response of salt stressed barley seedlings to phenyl urea. Plant Soil and Environment, 49 (4): 158-162.
Brachet, J. and Cosson, L. 1986. Changes in the total alkaloid content of Datura innoxia Mill subjected to salt stress. Journal of Experimental Botany, 37: 650-656.
Hadian, J., Esmaeili, H., Nadjafi, F. and Khadivi-Khub, A. 2014. Essential oil characterization of Satureja rechingeri in Iran. Industrial Crops and Products, 61: 403-409.
Hendawy, S. F. and Khalid, Kh. A. 2005. Response of sage (Salvia officinalis L.) plants to zinc application under different salinity levels. Journal of Applied Sciences Research, 1: 147-155.
Jamzad, Z. 1996. Satureja rechingeri (Labiatae) – a new species from Iran. Naturhistoriches Museum Wien, 98: 75-77.
Kaldenholff, R. and Fischer, M. 2006. Aquaporins in Plants. Acta Physiologica, 187: 169-176.
Khodakovskaya, M., Dervishi, E., Mahmood, M., Xu, Y., Li, Z. R., Watanabe, F. and Biris A. S. 2009. Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano, 3 (10): 3221-3227.
Khodakovskaya, M. V., De Silva, K., Biris A. S., Dervishi, E. and Villagarcia, H. 2012. Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano, 6 (3): 2128-35.
Maurel, C. 2007. Plant Aquaporins Novel Functions and Regulation Properties. FEBS Letters, 581: 2227-2236.
Meloni, D. A., Oliva, M. A., Martinez, C. A. and Cambraia, J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and gluthatione reductase in cotten under salt stress. Environmental and Experimental Botany, 49: 69-76.
Mirsa, A. and Strivastava, N. K. 2000. Influence of water stress on Japanese mint Journal of Herbs, Spices and Medicinal Plants, 7: 51-58.
Mohammad, M., Shibli, R., Ajlouni, M. and Nimri,L. 1998. Tomato root and shoot responses to salt stress under different levels of phosphorus nutrition. Journal of Plant Nutrition, 21 (8): 1667-1680.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Plant Physiology, 15: 473.
Najafi, F., Khavari-Nejad, R. A. and Siah Ali, M. 2010. The Effects of Salt Stress on Certain Physiological Parameters in Summer Savory (Satureja hortensis L.) Plants. Journal of Stress Physiology and Biochemistry, 6: 13-21.
Parida, A. K. and Das, A. B. 2002. Effect of NaCl stress on nitrogen and phosphorous metabolism in a true mangrove Bruguriera parviflora grown under hydroponic culture. Journal of Plant Physiology, 161: 921-928.
Philip, G. C., Keith, B., Masa, I. and Zettl, A. 2000. Extreme oxygen sensitivity of electronic properties of carbon nanotubes. Science, 287 (5459): 1801-1804.
Reich, E. and Schibli, A. 2006. High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants. Thieme Medical Publishers, New York, 197 pp.
Shao, H. B., Chu, L. Y. and Jaleel, C. A. 2008. Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies, 331 (3): 215-25.
Slinkard, K. and Singleton, V. L. 1977. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
Tripathi, S. H., Sonkar, S. K. and Sarkar, S. 2011. Growth stimulation of gram (Cicer arietinum) plant by water soluble carbon nanotubes. Nanoscale, 3 (3): 1176-1181.
Wang, X., Han, H., Liu, X., Gu, X., Chen, K. and Lu, D. 2012. Multi-walled carbon nanotubes can enhance root elongation of wheat (Triticum aestivum) plants. Journal of Nanoparticle Reserarch, 14: 841-851.
Zhishen, J., Mengcheng, T. and Jianming, W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64 (4): 555-559.
 
Volume 10, Issue 1
June 2018
Pages 47-58

Files

Share

How to cite

Statistics