Document Type : Research Paper
Authors
1
Research Assistant Professor, Seed and Plant Improvement Section, , Hamedan Agricultural and Natural Resources Research and Education Center, Hamedan, Iran
2
Former MSc Student, Department of Agricultural Biotechnology, Payame Noor University, Tehran Shargh Branch, Tehran, Iran
3
Associate of Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran Shargh Branch, Tehran, Iran
4
Instructor, Seed and Plant Improvement Section, , Hamedan Agricultural and Natural Resources Research and Education Center, Hamedan, Iran
Abstract
Achieving an efficient combination of sucrose and benzyl adenin (BA) concentration is one of the success factors in potato microtuber production. In this experiment in order to study the effect of different concentrations of BA and sucrose on plantlets growth traits and their production of microtubers, healthy plantlets of Arinda cultivar were treated with different concentrations of BA and sucrose in MS culture medium in vitro. This research was conducted as a factorial experiment based on a completely randomized design. BA was used as the first factor with the concentrations of 0 (control), 50, 100 and 150 μM, and the second factor was sucrose at three concentration levels (30, 45 and 60 grams per liter). Some plantlets growth traits including stolon length, leaf number, root fresh and dry weight, stem fresh and dry weight, number of microtuber, fresh microtuber weight and its dry matter percentage were measured. The results showed that the effect of BA on all traits was significant, while sucrose effect was significant on some of traits only. The effect of BA and sucrose interaction was significant only on stolon length and microtuber weight. In most of the growth traits, as well as the number of microtuber and its percentage of dry matter, the concentration of 50 μM BA had the better results compared to the two other concentrations (100 and 150 μM). Nevertheless, there were the same results between 150 µM BA and control treatment in the most traits as well as microtuber productions. Growth traits of plantlet and their yield improved by the change in sucrose concentration, from 30 to 60 grams per liter.
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