Effects of Culture Medium Type, Sucrose and Growth Regulator Combination on Gerbera Plantlets Rooting In Vitro

Document Type : Short Communication

Authors

1 Assistant Professor, Department of Ornamental Plants Biotechnology, University Jihad of Khorasan Razavi, Mashhad, Iran

2 Former MSc Student, Department of Ornamental Plants Biotechnology, University Jihad of Khorasan Razavi, Mashhad, Iran

Abstract

Gerbera is one of the most important cut flower and potted plant that normally propagated via tissue culture tecniques. For the suitable adaptation of plantlets in tissue culture, having a strong rooting system is crucial. So, in this study the effect of culture medium type, sucrose and hormonal combination on rooting of Gerbera plantlets was investigated. Factorial experiment was conducted in a completely randomized design with 3 factors and 4 replications. Before culturing, plantlets were immersed in GA3 (5 mg/l), GA3 (2.5 mg/l) + IAA (5 mg/l) and distilled water for 5 minutes and after that, they were cultured in ½ MS or modified White media. Also, in order to investigate the effect of sucrose on rooting of explants, the concentrations of 0 (control) and 30 g/l sucrose were used in the culture media. At the end of the experiment, different traits were measured. Results showed that the produced plantlet in the non-sucrose medium had the shorter roots and the use of sucrose in the culture medium increased root length by more than 2.5 times. Also, in the absence of sucrose, the primary and secondary root number fell sharply and the weight of plantlet reduced. Hormon-free ½ MS medium containing 30 g/l sucrose led to the production of maximum secondary root number and dry weight as well as an acceptable number of main root. So, for in vitro rooting of gerbera plantlets, application of hormon-free ½ MS medium supplemented with sucrose is recommended.

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Volume 12, Issue 1
June 2020
Pages 125-135

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