Effect of Explant Sampling Time and Culture Medium Conditions on Explant Establishment of Two Walnut Cultivars; Chandler and Jamal

Document Type : Research Paper

Authors

1 PhD Student, Department of Horticultural Sciences, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran

2 Professor, Department of Horticultural Sciences, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran

Abstract

Oxidation of exuded phenolic compounds into the culture media, culture medium browning and ceasing growth are the main obstacles in explants establishment during walnut micropropagation. This research was performed as a factorial experiment based on a completely randomized design with three factors including cultivar (Jamal and Chandler), shoot sampling time (May, July and September) and medium conditions (adding activated charcoal to the medium, placing cultures for 24 and 72 h in darkness, sub-culturing thrice per 24 h, twice per 72 h and once after 144 h and the control). Results of the variance analysis of the data related to the establishment of micro-cuttings in culture medium showed that cultivar, sampling time and culture medium conditions as well as their interactions, except the interaction between micro-cuttings and sampling time had significant effects on the Jamal and Chander cultivar micro-cuttings establishment. According to the results of the means comparison, regarding treatments including placement of cultures thrice per 24 hours and adding activated charcoal had a highest level of micro-cuttings establishment in culture medium having significant difference with the other treatments. On the other hand, the results showed a highly significant negative correlation (-0.86) between the micro-cuttings browning index and micro-cuttings establishment. In conclusion, the micro-cuttings establishment of Chandler cultivar had the higher rate compared to Jamal cultivar having statistically significant differences. Finally, it can be stated that collection of micro-cuttings in May and sub-culturing them thrice every 24 hours or adding activated charcoal showed the best results in Chandler and Jamal micro-cuttings establishment.  

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Volume 12, Issue 1
June 2020
Pages 137-152

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