Effect of Seed priming on Germination, Biochemical Indices and yield components of Seed in Different Cultivars of Safflower (Carthamus tinctorius L.) under Drought

Document Type : Research Paper

Authors

1 PhD Graduate, Department of Agronomy, Faculty of Agriculture, Islamic Azad University, Sciences and Research Branch, Tehran, Iran

2 Professor, Department of Agronomy, Faculty of Agriculture, Islamic Azad University, Sciences and Research Branch, Tehran, Iran

3 Assistant Professor, Department of Agronomy, Faculty of Agriculture, Islamic Azad University, Saveh Branch, Saveh, Iran

4 Associate Professor, Department of Agronomy, Faculty of Agriculture, Arak Branch, Islamic Azad University, Arak, Iran

Abstract

In order to investigate the effect of different methods of seed priming on germination indices, biochemical characteristics and yield component of three safflower cultivars under drought stress conditions, two experiment was carried out in the form of filed (as split factorial in randomized complete blocks design) and laboratory (as factorial based on completely randomized design) with three replications. The experimental factors included drought stress in two levels of complete irrigation during the growing season and drought stress at the stem elongation as the main factor and seed priming with osmopriming, hydropriming, hormopriming and control (without priming) and safflower cultivars included Isfahan, Sofeh and Goldasht as subplot factor. Simultaneous application of hydropriming and drought stress in Isfahan cultivar resulted in the highest root length (4.8 cm). Hydropriming and hormopriming increased the coefficient of velocity of germination in Isfahan cultivar 52.34% compared to control. Hydropriming increased the germination percentage by 11.33% compared to the control. The highest seed yield (100.72 g / m2) were obtained in Sofeh cultivar during complete irrigation and hydropriming application. The highest soluble carbohydrate amount (15.14mg/g FW) was related to the interaction between stress and no priming. Goldasht cultivar had the highest oil yield (24.93 g / m2) at the time of complete irrigation and hydropriming application. Usage of hydropriming during stress in Goldasht cultivar caused the highest amount of proline (10.08 mg/g FW). The interaction of hormopriming with drought stress causes the most activity of superoxide dismutase enzyme (56.1 mg protein/min).

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