Document Type : Short Communication
Authors
1
MSc, Department of Agricultural Biotechnology, Payame Noor University of Karaj, Karaj, Iran
2
Professor, Department of Agricultural Biotechnology, Payame Noor University of Karaj, Karaj, Iran
3
Associate Professor, Department of Tissue Culture, Agricultural Biotechnology Research Institute, Najaf Abad, Isfahan, Iran
4
Instructor, Departemant of Horticulture, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract
In this study, the effect of different disinfectant substances (70% Ethylic alcohol, sodium hypochlorite, Mercuric chloride, Cetrimide & chlorhexidine, Dettol, Cephalexin, Permanganate, Ridomil, Potassium phosphite) for contamination control in rhizome derived explants of Alstroemeria cv. ‘Balance’, were investigated. After culturing, explants pollution rates for different treatments, was measured daily. These experiments were implemented in a CRD design with 20 treatments and 3 replications. Analysis of variance (ANOVA) was done compared using LSD's test at 0.05 level of probability. It was revealed that disinfection with 70% Ethylic alcohol for one min, 3% sodium hypochlorite + 1 drop of Tween or dishwashing liquid for 20 min, 500 mg diluted Cephalexin in 400 milliliter sterile distilled water for a quarter; Et.Cl.Cf (C), was the most effective method in bacterial infection control. The procedure of disinfection with 70% Ethylic alcohol for one min, Dettol 1:40 diluted in sterile distilled water for 30 min, 3% sodium hypochlorite + 1 drop of Tween or dishwashing liquid for 10 min; Et.Dt.Cl (R), and disinfection method with 70% Ethylic alcohol for one min, Cetrimide and chlorhaxidine 1:10 diluted in sterile distilled water for 30 min, which the rhizomes being rinsed with sterile distilled water, which had been sterilized with 70% Ethylic alcohol for one min, 40% sodium hypochlorite + 1 drop of Tween or dishwashing liquid for 35 min; Et.Cl. (T) revealed the best effect on fungal contamination control. Disinfection with 70% Ethylic alcohol for one min, Cetrimide and chlorhaxidine 1:10 diluted in sterile distilled water for 30 min; Et.Cc. (L), was not effective in contaminations control.
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