Effect of Putrescine Application on the Development of Ovule and Subsequent Embryo Rescue of Two Iranian Stenospermic Grape (Vitis vinifera L.)

Document Type : Research Paper

Authors

1 Associate professor, Horticulture Urmia University, Urmia

2 Former graduate student of Horticulture Urmia University, Urmia

3 Research Assistant Professor Urmia University, Urmia

Abstract

Seedless grape cultivars (Vitis vinifera L.) are widely grown in Iran. Abortion of zygotic embryos in stenospermocarpic grapes largely limits the efficiency of breeding of seedless cultivars. The present study was designed to investigate effects of exogenously applied putrescine to the grapevines in field condition on ovule development and subsequent embryo rescue of two stenospermocarpic grape cultivars (Askary and Bidaneh Sefid). Concentrations of putrescine were 0, 2, 20 and 30mg/l. Length of berries and ovules were measured in Bidaneh Sefid after 20 days and Askary after 40 days of flower opening then, dissected out of ovules from berries were cultured in Nitsch & Nitsch medium containing 1µM GA3, 1µM NAA, 2 g/l activated charcoal, 20 g/l sucrose and 8 g/l agar. Evaluated characteristics were length of berries and ovules, browned, with callus and germinated ovules. The results showed that different concentrations of putrescine sprays were significant on length of berry and ovule, percentage of browned and germinated ovules at 1% level. Also percentages of ovules with callus were significant in different concentrations of putrescine at 5 %. Application of putrescine at 30 mg/l significantly increased length of berry and ovule, percentage of ovules with callus, germinated ovules and decreased browned ovules. Ovules germination at 30 mg/l concentration of putrescine was 30.018% in Askary cultivar and 24.412% in Bidaneh Sefid.

Keywords

Volume 4, Issue 2 - Serial Number 2
December 2013
Pages 89-98

Files

Share

How to cite

Statistics