The effect of different levels of molybdenum and nitrogen on some morphophysiological characteristics of spinach

Document Type : Research Paper

Authors

1 Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Professor of Hoticultural Sciences, Bu-Ali Sina University

3 Assistant Professor, Plant Production and Genetics Department, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, Iran

10.22084/ppt.2024.28466.2108

Abstract

Nitrate accumulation is a common problem in most leafy vegetables and it happens when the amount of nitrate absorption exceeds the amount consumed by the plant. This study was conducted as a factorial experiment in the form of a completely randomized design and in three replications. The first factor included adding nitrate (calcium nitrate) to Hoagland's standard solution at three levels of 0, 10 and 20%, and the second factor was adding different concentrations of molybdenum from sodium molybdate salt at four levels of 0, 0.5, 1.5 and 3 µM. The measured traits included traits of some growth characteristics, total phenol and flavonoids, ascorbic acid, spinach leaf nitrate. According to the obtained results, fresh and dry weight of leaf, dry weight of root and percentage of dry matter increased by increasing calcium nitrate concentration up to 20% and molybdenum application up to 3µM in nutrient solution. The simultaneous application of molybdenum and nitrogen had no significant effect on total phenolic, flavonoid and ascorbic acid traits, and the highest amount of these traits was observed in 3µM molybdenum. The highest amount of leaf nitrate (3247.4 mgkg-1FW) was observed in the treatment of 20% additional nitrate along with molybdenum. Based on the results, the use of molybdenum in nutrient solution had a reducing effect on nitrate accumulation in spinach leaf, therefore adding 3µM sodium molybdate to Hoagland nutrient solution in hydroponic system for spinach production is recommended to obtain the maximum amount of antioxidant compounds and the minimum amount of nitrate accumulation.

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