Optimized Culture Medium for In Vitro Growth of Grapevine Inflorescence

Document Type : Research Paper

Authors

1 PhD Student, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Professor, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

3 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

4 Professor, Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran

Abstract

In vitro inflorescence development and fruit production can provide a valuable tool for researchers investigating the precise physiological stages of these processes.In this study, inflorescences clusters from Bidaneh Sefid and Seedless grapes were cut off the young shoots growing on canes at spring. Half strength MS medium was used for in vitro cultivation of flowers. After cultivation of each individual inflorescence branches, they continued to development until berry ripening of fruits grown from these explants in vitro. The experiments were set as a factorial using completely randomized design with gibberellin in two levels; (0 and 2.89 μM), benzylaminopurin in three levels; (0, 2.5 and 4 μM) and auxin (indole butyric acid) in three levels; of (0, 2.5 and 4.9 μM) with three replicates for each treatment. Hormone type and concentration were impressive in successful growth of cultivated inflorescences. The cultivated inflorescences started growing, the flowers opened and eventually set fruits in media with 4.9 μM auxin and 4 μM cytokinin treatments. Auxin and cytokinin showed important roles as key hormones involved in flowers opening and fruit set in grapes, while gibberellin was non-significant. Bidaneh Sefid as a Seedless cultivar showed long term survival in vitro and more stable, during growth period of explants compared to  Seedless in acclimation to in vitro conditions. Control with no hormone treatment caused browning of flowers on the inflorescences in the medium and in some cases elucidation of browning in blooming flowers and stopped growth and eventually the flowers died.

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References

Amiri, M. E. and Fallahi, E. 2007. Influence of mineral nutrients on growth, yield, berry quality, and petiole mineral nutrient concentra­tions of table grapes. Journal of Plant Nutrition, 30: 463-470.
Baldwin, J. G. 1966. The effect of some cultural practices on nitrogen and fruitfulness in Sultana vine. American Journal of Enology and Viticulture, 17: 58-62.
Bessis, R., Charpentier, N., Hilt, C. and Fourioux, J. C. 2000. Grapevine fruit set: physiology of abscission zone. Australian Journal of Grape and Wine Research, 6: 125-130.
Berghoef, J. and Bruinsma, J. 1979. Flower development of Begonia franconis Liebm. II. Effects of nutrition and growth regulating substances on the growth of floral buds in vitro. Plant Physiology, 93: 345-357.
Boss, P. K., Vivier, M., Matsumoto, S., Dry, I. B. and Thomas, M. R. 2001. A CDNA from grapevine (Vitis vinifera L.), which shows homology to Agamous and Shatteproof, is not only expressed in flowers but also throughout berry development. Plant Molecular Biology, 45: 541-553.
Bouard, J. 1968. The influence of the carbohydrate and nutrient ele­ment content of the canes of the vine on the production of grapes. Potash Revenue, 29: 1-7.
CarmoVasconcelos, M., Greven, M., Chris, S., Winefield, M., Trought, C. T. and Raw, V. 2009. The flowering process of Vitisvinifera: A Review. American Journal of Enology and Viticulture, 60: 4-9.
Carmona, M. J., Martınez-Zapater, J. J. M. and Thomas, M. R. 2008. A molecular genetic perspective of reproductive development in grapevine. Journal of Experimental Botany, 59: 2579-2596.
De Jong, A. W. and Bruinsma, J. 1974. Pistil development in Cleome flowers. III. Effects of growth-regulating substances on flower buds of Clome iberidella Welw. ex Oliv. grown in vitro. Zeitschrift fűr Pflanzenphysiolie, 73: 142-151.
Feree, D. C., Bishop, B. L., Schupp, J. R., Tustin, D. S. and Cashmore, W. M. 2001. Influence of flower type, position in the cluster and spur characteristics on fruit set and growth of apple cultivars. Journal of Horticultural Science and Biotechnology, 76: 1-8.
Friend, A. P., Creasy, G. L., Trought, M. C. T. and Lang, A. 2003. Use of tagging to trace cap fall and development of individual Vitis vinifera L. cv. Pinot noir flowers. American Journal of Enology and Viticulture, 54: 313-317.
Hale, C. R. and Weaver, R. J. 1962. The effect of developmental stage on direction of translocation of photosynthate in Vitis vinifera. Hilgardia, 33: 89-141.
Imran, M. and Gurmani, Z. A. 2011. Role of macro and micro nutrients in the plant growth and development.Science, Technology and Development, 30: 30-37.
Keller, M. 2010. Managing grapevines to optimize fruit development in a challenging environment: a climate primer for viticulturists. Australian Journal of Grape and Wine Research, 16: 56-69.
Kieber, J. 2006. Cytokinins Regulators of cell division. In:Plant Physiology. Taiz, L. and Zeiger, E. (eds.), pp. 543-569. Sinauer Associates, Sunderland, Massachusetts.
Koblet, W. 1969. Translocation of photosynthate in vine shoots and influence of leaf area on quantity and quality of the grapes. Wein-Wiss, 24: 277-319.
Kozma, P. 2003. Exploration of flower types in grapes. InFloral Biology, Pollination and Fertilisation in Temperate Zone Fruit Species and Grape. pp: 75-226
Lebon, G., Brun, O., Mangé, C. and Clement, C. 2005. Photosynthesis of the grapevine (Vitis vinifera) inflorescence. Tree Physiology, 25:633-639.
Lombard, P. J., Cook, N. C. and Bellstedt, D. U. 2006. Endogenous cytokinin levels of table grape vines during spring budburst as influenced by hydrogen cyanamide application and pruning. Horticultural Science, 109: 92-96.
Longbottom, M., Dry, P. and Sedgley, M. 2004. Foliar application of molybdenum pre-flowering: Effects on yield of Merlot. Australian Grape Grower, 491: 36-39.
Loveys, B. R., Dry, P. R., Stoll, M. and McCarthy, M. G. 2000. Using plant physiology to improve the water use efficiency of horticulture crops. Acta Horticultural, 537: 187-197.
May, P. 2004. Flowering and Fruitset in Grapevines. Lythrum Press, Adelaide.
May, P. and Cellier, K. M. 1973. The fruitfulness of grape buds. II. The variability in bud fruitfulness in ten cultivars over four seasons. Annals Amelior Plant, 23: 13-26.
Mchughen, A. 1982. Induction organ generation in vitro: sepal-petal structures from tobacco buds. Canadian Journal Botany, 60: 845-849.
Mullins, M. G., Bouquet, A. and Williams, L. E. 1992. Biology of the Grapevine. Cambridge University Press, Cambridge, UK. pp: 239.
Okamoto, G., Tada, H., Suyama, A., Hayashi, Y. and Hirano, K. 2001. Effect of shoot vigor on the development of transmitting tissue and pollen tube growth in pistils of tetraploid grape, cv. Pione. Vitis, 40: 105-110.
Rastogi, R. and Sawhney, VK. 1987. The role of plant growth regulators, sucuros and pH in development of floral buds of tomato (Lycopersicon esculentum Mill.) culture in vitro. Journal Plant Physiology, 128: 285-295.
Rao, V., Venkatachalam, S., Natarajan, C. and Srinivasan, C. 1971. Uptake and movement of phosphorus (32P) in grapes. Vitis, 10: 103-106.
Robard, K., Prenzler, P. D., Tucker, G., Swatsitang, P. and Glover, W. 1999. Phenolic compounds and their role in oxidative processes in fruit. Journal Food Chemistry, 66: 401-436.
Schick, J. L. and Toivonen, P. M. A. 2002. Reflective traps at harvest reduce stem browning and improving fruit quality of cherries during subsequent storage. Postharvest Biology and Technology, 25: 117-121.
Srinivasan, C. and Mullins, M. G. 1981. Physiology of flowering in the grapevine. Review, American Journal of Enology and Viticulture, 32: 47-63.
Vasconcelos, M. C. and Castagnoli, S. 2000. Leaf canopy structure and vine performance. American Journal of Enology and Viticulture, 51: 390-396.
Yahyaouim, T., Barbier, M. and Bessis, R.1998. In vitro morphogenesis of grapevine (Vitis vinifera L.) inflorescence primordial, cvs Piont Noir and Chardonnay. Australian Journal of Grape and Wine Research, 4: 111-120.
Volume 7, Issue 2
July 2016
Pages 203-219

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