تأثیر سایکوسل بر فعالیت آنتی‌اکسیدانی و میزان مالون‌دی‌آلدئید در ارقام ماری و میشن زیتون( Olea europaea L.) تحت تنش خشکی

نوع مقاله: علمی - پژوهشی

نویسندگان

1 فارغ‌التحصیل کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

3 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

به‌منظور بررسی تأثیر سایکوسل بر برخی شاخص‌های بیوشیمیایی دو رقم زیتون (ماری و میشن) در شرایط تنش خشکی، آزمایش گلخانه‌ای با سه فاکتور شامل سه سطح سایکوسل (صفر، 500 و 1000 میلی‌گرم در لیتر)، سه سطح تنش خشکی (دور آبیاری فواصل 5، 10 و 15 روز یک‌بار) و دو رقم زیتون (ماری و میشن) به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار و در مدت چهار ماه انجام شد. در پایان آزمایش نتایج نشان داد که افزایش فواصل دور آبیاری، موجب افزایش فعالیت آنزیم‌های کاتالاز، آسکوربات پراکسیداز و گوایاکول پراکسیداز به­ترتیب 486/10، 129/10 و 879/17 میکرومول در دقیقه بر گرم وزن تر برگ در دور آبیاری 15 روز یک‌بار شد، همچنین میزان آنتوسیانین، فنل ‌کل و میزان مالون­دی­آلدئید در هر دو رقم افزایش یافت. تیمارهای مختلف سایکوسل نیز موجب افزایش فعالیت آنزیم کاتالاز و گوایاکول پراکسیداز و کاهش میزان مالون­دی­آلدئید در مقایسه با شاهد شدند. تیمار 500 میلی‌گرم در لیتر سایکوسل موجب کاهش معنی‌دار میزان آنتوسیانین برگ نسبت به شاهد شد (به­ترتیب 137/12 و 629/13 میکرومول بر گرم وزن تر برگ). رقم میشننسبت به رقم ماری مقاومت بیشتری به خشکی نشان داد. به‌طور­کلی نتایج نشان داد که استفاده از سایکوسل می‌تواند برخی از اثرات منفی ناشی از تنش خشکی در ارقام ماری و میشن زیتون را بهبود دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Cycocel on Antioxidative Activity and Malondialdehyde Content of،Mary and Mission Olive (Olea europaea L.) Cultivars Under Drought Stress

نویسندگان [English]

  • Vahid Akbari 1
  • Rasul Jalili Marandi 2
  • Alireza Farokhzad 3
1 M.Sc. Graduate, Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Associate Professors, Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
3 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

In order to investigate the effect of cycocel on some biochemical characteristics of two olive cultivars (Mary and Mission), a greenhouse experiment was conducted taking into account three factors of: cycocel at three levels (0, 500 and 1000 mg L-1), drought stress of three levels (irrigated at 5, 10 and 15 days intervals) and tow olive cultivars (Mary and Mission) in a factorial experiment based on randomized complete block design with three replicates for the lasted four months. In the end of experiment the results showed that increased periods of drought have increased catalase activity (10.486 µm min-1/g Fw-1), ascorbate peroxidase (10.129 µm min-1/g Fw-1) and guaiacol peroxidase (17.879 µm min-1/g Fw-1). Anthocyanin content, total phenols and malondialdehyde content in both cultivars increased with drought stress too. Also, cycocel treatments increased the catalase and guaiacol peroxidase activity and decreased the malondialdehyde content as compared with the control. The plants treated with 500 mg L-1 cycocel, showed significant decreased in the anthocyanin content (12.137 µm/g FW) in leaf compared with the control (13.629 µm/g FW). Mission cultivar showed higher resistance to drought stress than the Mary cultivar. Results suggested that cycocel treatment can neutralize some negative effects of drought stress in Mary and Mission cultivars of olive.

کلیدواژه‌ها [English]

  • Catalase enzyme
  • Ascorbate peroxidase
  • Guaiacol peroxidase
  • Total phenols

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