بررسی ترکیبات فنلی تراوش شده از ریزقلمه‌های تک‌گره گردو در محیط‌کشت مایع DKW

نوع مقاله : مقاله علمی-پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 استاد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

به‌منظور بررسی مقادیر برخی ترکیبات فنلی تراوش یافته از ریزقلمه‌های تک‌گره گردو به محیط‌کشت مایع (DKW) آزمایشی در قالب طرح بلوک‌های خرد‌شده با سه فاکتور شامل رقم در دو سطح (جمال و چندلر) و زمان تهیه ریزقلمه در چهار سطح (15 و 31 اردیبهشت، 15 تیر و 15 شهریور‌ماه) و زمان ارزیابی محیط‌کشت مایع در سه سطح (24،72 و 144 ساعت پس از کشت) در سه تکرار انجام شد. هم‌چنین در آزمایشی مستقل، ترکیبات فنلی تراوش شده به محیط‌کشت مایع در 24، 72 و 144 ساعت پس از کشت و ترکیب خالص ژوگلون به محیط‌کشت جامد اضافه شدند. نتایج نشان داد از لحاظ 14 ترکیب فنلی در شاخساره و محیط‌کشت شامل الاژیک‌ اسید، وانیلیک اسید، کوماریک اسید، کلروژنیک اسید، کافئیک اسید، جینتیسک اسید، فرولیک اسید، سیرینژیک اسید، سینامیک اسید، کاتچین، روتین و میریستین، ژوگلان و 1و4- نفتوکوئینون تفاوت معنی‌داری بین ارقام مشاهده نشد، امّا زمان تهیه ریزقلمه و زمان ارزیابی محیط‌کشت مایع تفاوت معنی‌داری نشان دادند. ترکیبات ژوگلان، الاژیک اسید، میریستین و وانیلیک اسید در مقادیر بالاتری نسبت به سایر ترکیبات فنلی در شاخساره سال جاری و ترکیبات ژوگلون، الاژیک اسید و میریستین بیش‌ترین تراوش را در محیط‌کشت مایع داشتند. اضافه کردن ترکیبات فنلی تراوش شده بعد از 144 ساعت در محیط‌کشت جامد ضعیف‌ترین رشد و کم‌ترین میزان تغییر وزن ریزقلمه را ایجاد کرد که این کاهش اختلاف معنی‌داری با دیگر تیمارها داشت. به‌طور‌کلی، احتمالاً سه ترکیب ژوگلون، الاژیک اسید و میریستین از موانع اصلی در استقرار کاملاً موفق ریزقلمه‌های دو رقم جمال و چندلر هستند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of Phenolic Compounds Exuded from Single Node Microcuttings of Walnut into Liquid Culture Medium DKW

نویسندگان [English]

  • Abdollah Ehteshamnia 1
  • Mansour Gholami 2
1 PhD Student, Department of Horticulture Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran
2 Professor, Department of Horticulture Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran
چکیده [English]

In order to study the amounts of some phenolic compounds exuded from single node walnut explants in liquid medium (DKW), this experiment was performed in the form of a split units in time experiment based on split block design with three factors including cultivar in two walnut cultivars namely Chandler and Jamal, time of sampling in four levels  including May 5 th, May 21st, July 5th and September 5th and the time of liquid culture assessment in three levels (24, 72 and 144 hours after the culture) using HPLC with three replicates. In independent experiment, phenolic compounds exuded in liquid medium 24, 72 and 144h after the culture and juglone were added to solid medium. The results showed that in terms of the 14 phenolic compounds (Ellagic, Vanillic, Coumaric, Chlorogenic, Caffeic, Gentistic Ferulic Syringic Cinamic acid, Catechin, Rutin and Myricetin, Juglone and 1,4 Naphthoquinone) in shoots and medium the cultivars were not significantly different but the time of sampling and liquid culture assessment showed significant differences between the cultivars. The results showed that Juglone, Myricetin, Ellagic and Vanillic acid were present in the current year shoots in higher amounts than other phenolic compounds and Juglone, Ellagic acid and Myricetin had the highest leakage in the culture medium. Adding exuded phenolic compounds after 144h in the solid medium had the lowest growth and fresh weight change of microcutting and significant difference with other treatments.The over all results of this study showed that Juglone, Ellagic acid and Myricetin are possibly the main obstacles to the fully succeed microcuttings establishment of two Jamal and Chandler cultivars.

کلیدواژه‌ها [English]

  • Juglone
  • Jamal
  • Chandler
  • Browning
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