عنوان مقاله [English]
Spearmint (Mentha spicata L.) is one of the most important medicinal plants of the mint family that have numerous applications in food, cosmetical and pharmaceutical industries due to its valuable medicinal properties. In this research, the change in essential oil content and composition of fresh leaf and flower of spearmint cultivated in Hamidieh and Shoshtar locations during five months on 2012 were studied. Fresh leaf samples were harvested on May, July, August, September and October. Fresh flower samples were harvested on July, August and September. The essential oil was extracted by Clevenger apparatus base on water distillation and analyzed by GC and GC-MS. Result showed that the harvest time had a significant effect on essential oil content of leaf and flower of both locations. The highest essential oil content of leaf (0.74%) and flower (2.84%) of Hamidieh samples were recorded on July and August, respectively and on August for Shoshtar samples (0.98 and 2.07%). The lowest essential oil content of leaf (0.26 and 0.42%) and flower (1.52 and 1.64%) were observed on May and July for both locations, respectively. In Hamidieh samples, the highest (69.60%) and lowest (51.92%) amounts of carvone of leaf were observed on October and May, respectively and the highest (63.80%) and lowest (53.33%) amounts of carvone of flower were also recorded on September and August, respectively. In Shoshtar samples, the highest carvone amount of leaf (64.03%) and flower (58.95%) was obtained on July and August, respectively and its lowest (60.34 and 54.26%) amount was observed on September. The highest limonene amount of leaf (23.30%) and flower (30.87%) was observed on July and August for Hamidieh samples and on August (20.99%) and September for Shoshtar (28.52%) samples, respectively.
امیدبیگی، ر. 1386. تولید و فرآوری گیاهان دارویی. جلد دوم، چاپ چهارم با بازنگری کامل، انتشارات آستان قدس رضوی، 438 صفحه.
سلطانی، ف.، شریفی، م.، خواجه، خ. و یوسفزادی، م.1387. بررسی ترکیبات اسانس، فعالیت آنزیم منتون ردوکتاز و فعالیت ضدمیکروبی گونه Mentha piperita در دو مرحله از رشد. مجله زیستشناسی ایران، 21 (5):70-62.
Adams, R. P. 2007. Identification of essential oil components by gas chromatography/mass spectroscopy. Allured: Carol Stream, IL, USA.
Akbari, M. T., Esmaeili, A., Zarea, A. H., Saad, N. and Bagheri, F. 2010. Chemical composition and antibacterial activity of essential oil from leaves, stems and flowers of Prangos ferulacea (L.) Lindl. grown in Iran. Bulgarian Chemical Communications, 42 (1): 36-39.
Chamorro, E. R., Ballerini, G., Sequeira, A. F., Velasco, G. A. and Zalazar, M. F. 2008. Chemical composition of essential oil from Tagetes minuta L. leaves and flowers. Journal of the Argentine Chemical Society, 96 (1-2): 80-86.
Chauhan, R. S., Kaul, M. K., Shahi, A. K., Kumar, A., Ram, G. and Tawa, A. 2009. Chemical composition of essential oils in Mentha spicata L. accession [IIIM (J) 26] from North-West Himalayan region, India. Industrial Crops and Products, 29: 654-656.
Dudai, N., Larkov, O., Ravid, U., Putievsky, E. and Lewinsohn, E. 2001. Developmental control of monoterpene content and composition in Micromeria fruticosa (L.) Druce. Annals of Botany, 88: 349-354.
Gomes, P. C. S., Oliveira, H. R. C., Vicente, A. M. S. and Ferreira, M. F. 2006. Production, transformation and essential oils composition of leaves and stems of lemon verbena [Aloysia triphylla (L’Herit.) Britton] grown in Portugal. Revista Brasileira de Plantas Medicinais, 8: 130-135.
Hadjiakhoondi, A., Aghel, N., Zamanizadeh-Nadgar, N. and Vatandoost, H. 2000. Chemical and biological study of Mentha spicata L. essential oil from Iran. Daru, 8 (1 and 2): 19-21.
Jaafar, F. M., Osman, Ch. P., Ismail, N. H. and Awang, Kh. 2007. Analysis of essential oils of leaves, stems, flowers and Rhizomes of Etlingera elatior (jack) r. M. Smith. The Malaysian Journal of Analytical Sciences, 11 (1): 269-273.
Jia, P., Liu, H., Gao, T. and Xin, H. 2013. Glandular trichomes and essential oil of Thymus quinquecostatus. The Scientific World Journal, Article ID 387952, 8 pages. doi:10.1155/2013/387952.
Kofidis, G., Bosabalidis, A. and Kokkini, S. 2004. Seasonal variations of essential oils in a linalool-rich chemotype of Mentha spicata grown wild in Greece. Journal of Essential Oil Research, 16: 469-472.
Lim, S. S., Jang, J. M., Park, W. T., Uddin, M. R., Chae, S. C., Kim, H. H. and Park, S. U. 2013. Chemical composition of essential oil from flower and leaf of Korean mint (Agastache rugosa), Asian Journal of Chemistry, 25 (8): 4361-4363.
Omar, N. A., El- Sayed, Z. I. A. and Romeh, A. A. 2009. Chemical constituents and biocidal activity of the essential oil of Mentha spicatal. grown in Zagazigregion, Egypt. Research Journal of Agriculture and Biological Sciences, 5 (6): 1089-1097.
Rao, B. R. R. 1999. Biomass and essential oil yields of cornmint (Mentha arvensis L. f. piperascens Malinvaud ex Holmes) planted in different months in semi-arid tropical climate. Industrial Crops and Products, 10: 107-113.
Santos, V. M. C. S., Pinto, M. A. S., Bizzo, H. and Deschamps, C. 2012. Seasonal variation of vegetative growth, essential oil yield and composition of menthol mint genotypes at Southern. Brazilian Bioscience Journal, 28 (5): 790-798.
Telci, I. and Sahbaz, N. 2005. Variation of yield, essential oil and carvone contents in clones selected from carvone-scented landraces of Turkish Mentha species. Journal of Agronomy, 4 (2): 96-102.
Telci, I., Demirtas, I., Bayram, E., Arabaci, O. and Kacar, O. 2010. Environmental variation on aroma components of pulegone/piperitone rich spearmint (Mentha spicata L.). Industrial Crops and Products, 32: 588-592.
Telci, I., Kacar, O., Bayram, E., Arabac, O., Demirtas, I., Yılmaz, G., Ozcan, I., Sönmez, C. and Göksu, E. 2011. The effect of ecological conditions on yield and quality traits of selected peppermint (Mentha piperita L.) clones. Industrial Crops and Products, 34: 1193-1197.
Toncer, O., Karaman, S. and Diraz, E. 2010. An annual variation in essential oil composition of Origanum syriacum from Southeast Anatolia of Turkey. Journal of Medicinal Plants Research, 4 (11): 1059-1064.
Werker, E., Putievsky, E., Ravid, U., Dudai, N. and Katzir, I. 1993. Glandular hairs and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Annals of Botany, 71: 43-50.
YesilCeliktas, O., HamesKocabas, E. E., Bedir, E., Vardar, S. F., Ozek, T. and Baser, K. H. C. 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry, 100: 553-559.
Zheljazkov, V. D., Cantrell, C. L., Tekwani, B. and Khan, S. I. 2008. Content, composition, and bioactivity of the essential oils of three basil genotypes as a function of harvesting. Journal of Agricultural and Food Chemistry, 5: 380-385.
Zheljazkov, V. D., Cerven, V., Cantrell, Ch. L., Ebelhar, W. M. and Horgan, T. 2009. Effect of nitrogen, location, and harvesting stage on peppermint productivity, oil content, and oil composition. Hortscience, 44 (5): 1267-1270.
Zheljazkov, V. D., Cantrell, C. L., Astatkie, T. and Hristov, A. 2010 (a). Yield, content, and composition of peppermint and spearmints as a function of harvesting time and drying. Journal of Agricultural and Food Chemistry, 58: 11400-11407.
Zheljazkov, V. D., Cantrell, C. L., Astatkie, T. and Ebelhar, M. W. 2010 (b). Productivity, oil content, and composition of two spearmint species in Mississippi. Agronomy Journal, 102 (1): 129-133.
Znini, M., Bouklah, M., Majidi, L., Kharchouf, S., Aouniti, A., Bouyanzer, A., Hammouti, B., Costa, J. and Al-Deyab, S. S. 2011. Chemical composition and inhibitory effect of Mentha spicata essential oil on the corrosion of steel in molar hydrochloric acid. International Journal of Electrochemical Science, 6: 691-704.