عنوان مقاله [English]
Tissue culture methods are widely used for crop breeding specialty for the selection of salt-tolerant plants. Salinity is one of the major abiotic stresses affecting agricultural production worldwide. In this study, the effect of different levels of salinity on some physiological and cells-growth characteristics in the cells of three potato cultivars (Agria, Marfona and Sante) in suspersion culture was studied. Calli were produced from the stem internodes of plants cultured on the semi-solid MS media containing 2 mg L-1 2,4-D and 0.4 mg L-1 Kinetin. Growing cells were then sub-cultured on the same culture media followed by transferring them into the liquid media containing 0, 50, 100, 150, 200 and 250 mM NaCl. Study was performed in a factorial experiment based on a completely randomized design with three replications. The amount of proline, total soluble carbohydrate, total soluble protein and the concentration of Na+ and K+ as well as the fresh and dry weight of the cells were measured. The results showed that by increasing salinity, the amount of proline, total soluble carbohydrates and total soluble protein as well as dry weight and Na+ concentration of the cells were increased, while their fresh weight and K+ concentration decreased. Three potato cultivars had different reactions to different levels of salinity. Sante cultivar has the highest accumulation of proline, total soluble carbohydrates, total soluble protein and potassium. This indicates that the above cultivar is more to lerant to salinity than the other cultivars.
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