اثر سطوح مختلف تنش خشکی بر تولید شیکوریک اسید در کاهوی موجدار (Lactuca undulata)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

2 دانشیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

3 استادیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

چکیده

کاهوی موجدار (. (Lactuca undulata Ledebگیاهی یک‌ساله از خانواده Asteraceae است. یکی از ترکیبات مهم این گیاه شیکوریک اسید است که در صنایع دارویی و غذایی از اهمیت زیادی برخوردار است. مطالعه حاضر به‌منظور بررسی اثر تنش خشکی بر رشد و برخی خصوصیات فیزیولوژیکی و بیوشیمیایی گیاه کاهوی موجدار انجام شده است. به این منظور گیاهچه‌های 45 روزه به‌مدت 7 روز تحت تیمار خشکی با پتانسیل 0، 3/0-، 6/0-، 9/0- و 2/1- بار (با استفاده از پلی‌اتیلن گلیکول 4000) استفاده شد. نتایج حاصل نشان داد که با افزایش تنش خشکی گیاهان به تدریج پژمرده شدند. بیش‌ترین میزان وزن خشک و تر ریشه و برگ، وزن کل گیاهچه‌ها، فنل کل ریشه و برگ، فلاونوئید کل برگ، قند محلول ریشه و برگ، پروتئین محلول ریشه، و فعالیت آنزیم پراکسیداز ریشه در تیمار با پتانسیل خشکی 6/0- بار مشاهده شد. بالاترین میزان فلاونویید کل ریشه و فعالیت پراکسیداز برگ در تیمار با پتانسیل خشکی 9/0- بار مشاهده شد. میزان پروتئین محلول ریشه با افزایش تنش خشکی به‌تدریج افزایش یافت. میزان فعالیت آنزیم کاتالاز در هر دو نمونه ریشه و برگ با افزایش تنش خشکی کاهش معنی‌داری را نسبت به شاهد نشان داد. نتایج حاصل از آنالیز HPLC نشان داد که تنش خشکی با پتانسیل 6/0- و 9/0- بار سبب افزایش معنی‌دار در میزان شیکوریک اسید ریشه نسبت به شاهد شده است. درمجموع داده‌های حاضر نشان داد که تیمار خشکی با پتانسیل 6/0- بار سبب بهبود فاکتورهای رشد و افزایش میزان متابولیت‌های ثانویه آن می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Impact of Different Levels of Drought Stress on Cichoric Acid Production in Lactuca undulata

نویسندگان [English]

  • Abdolah Saghali 1
  • Mahnaz Aghdasi 2
  • Seyed Mohammad Fatemi 3
1 MSc Student, Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
2 Associate Professor, Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
3 Assistant Professor, Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
چکیده [English]

Lactuca undulata is an annual plant belonging to the Asteraceae family. Cichoric acid is one of the most important phytochemical compounds, which is used in the pharmaceutical and food industries. The main objective of the present study was to investigate the effects of drought stress on the morphological and physiological characteristics of Lactuca undulata. For this purpose, 45 days old seedlings were subjected to drought stress at water potential of 0, -0.3, -0.6, -0.9, and -1.2 bar. Polyethylene glycol 4000 was used to create and applydrought stress. The current results showed that by increasing drought stress, the plants gradually withered. The highest level of root and leaf dry and fresh weight, seedling total weight, root and leaf total phenol, leaf total flavonoid, root and leaf soluble sugar, root soluble protein, and root peroxidase enzyme activity were obtained under drought stress with water potential of -0.6 bar. However, the highest amount of root total flavonoid and leaf peroxidase enzyme activity were observed under drought stress with a potential -0.9 bar. The amount of root soluble protein gradually increased with increasing drought stress. But, root and leaf catalase activity showed a significant decrease by increasing drought stress, compared to control. Data from HPLC analysis showed that the highest amount of cichoric acid was obtained after drought stress with water potential of -0.6 and -0.9 bar. In conclusion, the present data showed that drought stress with a potential of -0.6 bar improves the growth factor and the amount of its secondary metabolites.

کلیدواژه‌ها [English]

  • Water potential
  • Secondary metabolite
  • Caffeic acid
  • Growth
  • Antioxidant activity
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