نوع مقاله : مقاله علمی-پژوهشی
نویسندگان
1 دانش‌آموخته کارشناسی‌ارشد علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد
2 استاد گروه علوم‌باغبانی و عضو پیوسته گروه گیاهان‌زینتی پژوهشکده علوم‌گیاهی دانشکده کشاورزی، دانشگاه فردوسی مشهد ، مشهد
3 استادیار گروه علوم باغبانی دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد
4 استادیار گروه گیاهپزشکی دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Contamination in tissue culture is a common problem that induces the elimination of some or whole explants in tissue culture medium. The aim of this study was to eliminate or decreasing the contamination in tissue culture. In stage one, for determination of the best anti-fungi material and suitable concentration for spore germination inhibitory of Penicillium digitatum, inhibitory spore germination pre-test on MS medium was examined. Result showed that the best treatments for spore germination inhibitory were cinnamon essential oil with 50 and 75 ppm and methyl and propyl paraben at concentration of 600 and 225 ppm. In stage two, hyphe growth of Penicillium digitatum in selected treatment was evaluated. Result emphasis that in the selected treatment, spore germination was inhibited better than hyphe growth. The highest and lowest hyphe growth was related to cinnamon essential oil and methyl paraben treatment. In stage three, apple plantlets with essential oil and paraben in various concentrations were cultured on MS medium. Result showed that in the whole treatment, increasing the concentration of anti-fungal materials reduced growth properties of plants. The best growth of apple plantlets was observed in cinnamon essential oils. In stage four, the effect of paraben and essential oils with spore of Penicillium digitatum in MS medium on growth of plantlets was evaluated. Result showed that although methyl and propyl paraben at concentration of 600 and 225 ppm controlled spore germination very well but contamination in MS medium appeared. Therefore, the best treatment was cinnamon essential oil at concentration of 50 and 75 ppm.
کلیدواژه‌ها [English]
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