تأثیر پرایمینگ بذر با کیتوزان بر جوانه‌زنی، روابط یونی و خصوصیات بیوشیمیایی نخود تحت تنش شوری

نوع مقاله : مقاله علمی-پژوهشی

نویسندگان

1 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ولی‌عصر (عج) رفسنجان، رفسنجان، ایران

2 دانشجوی کارشناسی ‌ارشد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ولی‌عصر (عج) رفسنجان، رفسنجان، ایران

3 استاد گروه زراعت، دانشکده‌ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

برای ارزیابی تأثیر پرایمینگ (شاهد= بدون پرایمینگ)، آب مقطر، کیتوزان 1/0 درصد و کیتوزان 2/0 درصد بر جوانه ­زنی و رشد گیاهچه نخود تحت تنش شوری (صفر، 4، 6 و 8 دسی‌زیمنس بر متر)، آزمایشی در شرایط محیطی کنترل شده انجام شد. نتایج نشان داد که تنش شوری موجب کاهش درصد و سرعت جوانه ­زنی، شاخص ویگور، طول و وزن خشک ساقه ­چه و ریشه ­چه، میزان پتاسیم و نسبت پتاسیم به سدیم گردید، درحالی‌که میزان سدیم، محتوای پرولین، کربوهیدرات­های کل و مالون دی­آلدئید را افزایش داد. پرایمینگ بذر با کیتوزان درصد و سرعت جوانه ­زنی را نسبت به سایر تیمارهای پرایمینگ افزایش داد. در تنش شوری dS/m 8، بذرهای پرایم شده با کیتوزان شاخص ویگور، طول ساقه ­چه، طول ریشه ­چه و وزن خشک ساقه­ چه بیشتری نسبت به شاهد داشتند. بیشترین محتوای پتاسیم در پرایم با کیتوزان 2/0 درصد مشاهده شد. همچنین با افزایش شوری، کیتوزان سبب کاهش میزان سدیم و مالون دی آلدئید و افزایش نسبت پتاسیم به سدیم، محتوای پرولین و کربوهیدرات کل گیاهچه ­ها گردید. به این ترتیب می­توان اظهار داشت که تأثیر عمده پیش‌تیمار بذر نخود با کیتوزان بر رشد گیاهچه بود و تأثیر قابل­ملاحظه‌ای بر افزایش درصد جوانه ­زنی نداشت. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of Chitosan Seed Priming on Germination, Ion Relations and Biochemical Characteristics of Chickpea Under Salinity Stress

نویسندگان [English]

  • Batool Mahdavi 1
  • Hossein Safari 2
  • Seyed Ali Mohammad Modarres-Sanavy 3
1 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
2 MSc Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
3 Professor, Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

In order to study the effects of priming (control (non-primed), distilled water, 0.1% chitosan and 0.2% chitosan) on seed germination and growth of chickpea seedling, under salinity stress (0, 4, 6 and 8 dS/m) an experiment was conducted in controlled-environment conditions. Results showed salinity stress reduced germination percentage and rate, vigor index, length and dry weights of shoots and roots, K concentration and K/Na ratio, whereas increased Na concentration, proline, total carbohydrate and malondialdehyde content. Seed priming with chitosan increased germination percentage and rate compared to other priming treatments.Vigor index, shoot length, root length and dry weights of shoots were higher in chitosan primed seed compared to control under salinity stress 6 dS/m. The highest K concentration was observed in seed primed with 0.2% chitosan. Also, with the increasing salinity, chitosan decreased Na concentration and malondialdehyde and increased K/Na ratio, proline and total carbohydrate content. Thus, it suggests that main effect priming the chickpea seeds with chitosan was on seedling growth and it don’t effect on increasing germination percentage. 

کلیدواژه‌ها [English]

  • Malondialdehyde
  • Proline
  • Seed pretreatment
  • Total carbohydrate

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