تأثیر پرایمینگ بذر با کیتوزان بر جوانه‌زنی، روابط یونی و خصوصیات بیوشیمیایی نخود تحت تنش شوری

نوع مقاله: علمی - پژوهشی

نویسندگان

1 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ولی‌عصر (عج) رفسنجان، رفسنجان، ایران

2 دانشجوی کارشناسی ‌ارشد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ولی‌عصر (عج) رفسنجان، رفسنجان، ایران

3 استاد گروه زراعت، دانشکده‌ کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

برای ارزیابی تأثیر پرایمینگ (شاهد= بدون پرایمینگ)، آب مقطر، کیتوزان 1/0 درصد و کیتوزان 2/0 درصد بر جوانه ­زنی و رشد گیاهچه نخود تحت تنش شوری (صفر، 4، 6 و 8 دسی‌زیمنس بر متر)، آزمایشی در شرایط محیطی کنترل شده انجام شد. نتایج نشان داد که تنش شوری موجب کاهش درصد و سرعت جوانه ­زنی، شاخص ویگور، طول و وزن خشک ساقه ­چه و ریشه ­چه، میزان پتاسیم و نسبت پتاسیم به سدیم گردید، درحالی‌که میزان سدیم، محتوای پرولین، کربوهیدرات­های کل و مالون دی­آلدئید را افزایش داد. پرایمینگ بذر با کیتوزان درصد و سرعت جوانه ­زنی را نسبت به سایر تیمارهای پرایمینگ افزایش داد. در تنش شوری dS/m 8، بذرهای پرایم شده با کیتوزان شاخص ویگور، طول ساقه ­چه، طول ریشه ­چه و وزن خشک ساقه­ چه بیشتری نسبت به شاهد داشتند. بیشترین محتوای پتاسیم در پرایم با کیتوزان 2/0 درصد مشاهده شد. همچنین با افزایش شوری، کیتوزان سبب کاهش میزان سدیم و مالون دی آلدئید و افزایش نسبت پتاسیم به سدیم، محتوای پرولین و کربوهیدرات کل گیاهچه ­ها گردید. به این ترتیب می­توان اظهار داشت که تأثیر عمده پیش‌تیمار بذر نخود با کیتوزان بر رشد گیاهچه بود و تأثیر قابل­ملاحظه‌ای بر افزایش درصد جوانه ­زنی نداشت. 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Chitosan Seed Priming on Germination, Ion Relations and Biochemical Characteristics of Chickpea Under Salinity Stress

نویسندگان [English]

  • Batool Mahdavi 1
  • Hossein Safari 2
  • Seyed Ali Mohammad Modarres-Sanavy 3
1 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
2 MSc Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
3 Professor, Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

In order to study the effects of priming (control (non-primed), distilled water, 0.1% chitosan and 0.2% chitosan) on seed germination and growth of chickpea seedling, under salinity stress (0, 4, 6 and 8 dS/m) an experiment was conducted in controlled-environment conditions. Results showed salinity stress reduced germination percentage and rate, vigor index, length and dry weights of shoots and roots, K concentration and K/Na ratio, whereas increased Na concentration, proline, total carbohydrate and malondialdehyde content. Seed priming with chitosan increased germination percentage and rate compared to other priming treatments.Vigor index, shoot length, root length and dry weights of shoots were higher in chitosan primed seed compared to control under salinity stress 6 dS/m. The highest K concentration was observed in seed primed with 0.2% chitosan. Also, with the increasing salinity, chitosan decreased Na concentration and malondialdehyde and increased K/Na ratio, proline and total carbohydrate content. Thus, it suggests that main effect priming the chickpea seeds with chitosan was on seedling growth and it don’t effect on increasing germination percentage. 

کلیدواژه‌ها [English]

  • Malondialdehyde
  • Proline
  • Seed pretreatment
  • Total carbohydrate
Abdul-baki, A. A. and Anderson, J. D. 1970. Viability and leaching of sugars from germinating barely. Crop Science, 10: 31-34.

Agrawal, R. L. 2004. Seed technology. Oxford and IBH publishing Co. LTD. New Dehli, pp: 350.

Allen, G. J., Wyn Jones, R. G. and Leigh, R. A. 1995. Sodium transport measured in plasma membrane vesicles isolated from wheat genotypes with differing K+/Na+ discrimination traits. Plant Cell and Environment, 18: 105-115.

Basra, S. M. A., Afzal, I., Rashid, R. A. and Hameed, A. 2005. Inducing salt tolerance in wheat by seed vigor enhancement techniques. International Journal of Biology and Biotechnology, 2: 173-179.

Bates, L. S., Waldern, R. P. and Teave, I. D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207.

Bautista-Banos, S., Hernandez-Lopez, M. and Bosquez- Molina, E. 2004. Growth inhibition of select fungi by chitosan and plant extracts. Mexican Journal of Phytopathology, 22: 178-186.

Bhamburdekar, S. B. and Chavan, P. D. 2011. Effect of some stresses on free proline content during pigeonpea (Cajanas cajan) seed germination. Journal of Stress Physiology & Biochemistry, 7 (3): 235-241

Bohnert, K. H., Nelson, D. E. and Jensen, R. G. 1995. Adaptations to environment stresses. Plant Cell, 7: 1099-1111.

Cho, M. H., No, H. K. and Prinyawiwatkul, W. 2008. Chitosan treatments affect growth and selected quality of sunflower sprouts. Journal of Food Science, 73 (1): 570-577.

De Vos, C., Schat, H., De Waal, M., Vooijs, R. and Ernst, W. 1991. Increased to copper-induced damage of the root plasma membrane in copper tolerant silene cucubalus, Plant Physiology, 82: 523-528.

Dzung, N. A. 2011. Enhancing Crop Production with Chitosan and Its Derivatives. In : Kim, S. K. eds. Chitin, Chitosan, Oligosaccharides and Their Derivatives, pp: 643.

Farouk, S., Ghoneem, K. M. and Ali Abeer, A. 2008. Induction and Expression of systematic risistance to downy mildew disease in cucumber plant by elicitors. Egyptian Journal of Phytopathology, 36 (1-2): 95-111.

Grime, J. P. and Campbell, B. D. 1991. Growth rate, habitat productivity, and plant strategy as predictors of stress response. In: Mooney, H. A., Winner, W. E., Pell, E. J. and Chu, E. eds. Response of Plants to Multiple Stresses, pp. 143-159

Guan, Y. J., Hu, J., Wang, X. J. and Shao, C. X. 2009. Seed priming with Chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University-Science B, 10: 427-433.

Hadwiger, L. A., Klosterman, S. J. and Choi, J. J. 2002. The mode of action of Chitosan and its oligomers in inducing plant promoters and developing disease resistance in plants, In: Suchiva, K., Chandrkrachang, S., Methacanon, P. and Peter M. G. eds. Advances in Chitin Science,  pp. 452-457

Hampton, J. G. and Tekrony, D. M. 1995. Handbook of Vigor Test Methods. 3rd edition. The international Seed Testing Association, Zurich, Switzerland, pp: 117.

Hasegawa, P. M., Bresson, R. A., Zhu, J. K. and Bohnert, H. J. 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology, 51: 463-499.

Khajeh, M., Powell, A. A. and Bingham, I. J. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Science and Technology, 31: 715-725.

Khan, M. A. and Gulzar, S. 2003. Germination responses of Sporobolus ioclados, a saline desert grass. Journal of Arid Environments, 55: 453-464.

Khan, M. N., Siddiqui, M. H., Mohammad, F., Naeem, M. and Khan, M. M. A. 2010. Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation. Acta Physiologiae Plantarum, 32: 121-132.

Lianju, M., Yueying, L., Cuimei, Y., Yan, W., Xuemei, L., Na, L., Qiang, C. and Ning, B. 2011. Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress. Protoplasma, 249: 393-399

McDonald, M. B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology, 27 (1): 177-237.

Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell and Environment, 25: 239-250.

Munns, R. and Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59: 651-681.

Murakeozy E. P., Nagy, Z., Duhaze, C., Bouchereau, A. and Tuba, Z. 2003. Seasonal changes in the levels of compatible osmolytes in three halophytic species of inland saline vegetation in Hungary. Journal of Plant Physiology, 160: 395-401.

No, H. K., Lee, K. S., Kim, I. D., Park, M. J., Kim, S. D. and Meyers, S. P. 2003. Chitosan treatment affects yield, ascorbic acid content, and hardness of soybean sprouts. Journal of Food Science, 68: 680-685.

Patel, P. R., Kajal, S. S., Patel, V. R., Patel, V. J. and Khristi, S. M. 2010. Impact of salt stress on nutrient uptake and growth of cowpea. Brazilian Journal of Plant Physiology, 22 (1): 43-48.

Ruan, S. l., Xue, Q. Z. and Tylkowska, K. 2002. The influence of priming on germination of rice (Oryza sativa L.) seeds and seedling emergence and performance in flooded soil. Seed Science  and Technology, 30: 61-67.

Ruan, S. L. and Xue, Q. Z. 2002. Effects of chitosan coating on seed germination and salt-tolerance of seedlings in hybrid rice (Oryza sativa L.). Acta Agronomica Sinica, 28 (6): 803-808 (in Chinese).

Shao, C. X., Hu, J., Song, W. J. and Hu, W. M. 2005. Effects of seed priming with chitosan solutions of different acidity on seed germination and physiological characteristics of maize seedling. Journal of Zhejiang University (Agriculture & Life Sciences), 31 (6): 705-708 (in Chinese).

Sun, W. Y., Wang, H. and Huang, J. C. 2001. The effect of external betaine on membrane lipid peroxidation of wheat seedling under water stress. Acta Botanica Boreali-Occidentalia Sinica, 21: 487-491.

Trotel, P., Bouchereau, A., Niogret, M. F. and Larher, F. 1996. The fate of osmo-accumulated proline in leaf discs of Rape (Brassica napus L.) incubated in a medium of low osmolarity. Plant Science, 118: 31-45.

Turner, N. C., Wright, G.  C. and Siddique, K. H. M. 2001. Adaptation of grain legumes (Pulses) to water limited environments. Advances in Agronomy, 71: 193-231.

Woodward, A. J. and Bennett, I. J. 2005. The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tissue and Organ Culture, 82: 189-200.

Xue, Y. S., Wen Qing, Z., Wei, X. and Qing, W. 2002. Effect of chitosan as seed coating on seed germination and seedling growth and several physiological and biochemical indexes in rapeseed. Plant Physiology Communications, 38 (3): 225-227.

Zeng, D., Luo, X. and Tu, R. 2012. Application of bioactive coatings based on chitosan for soybean seed protection. International Journal of Carbohydrate Chemistry, 1-5.

Zhou, Y. G., Yang, Y. D., Qi, Y. G., Zhang, Z. M., Wang, X. J. and Hu, X. J. 2002. Effects of chitosan on some physiological activity in germinating seed of peanut. Journal of Peanut Science, 31: 22-25.