نوع مقاله : مقاله علمی-پژوهشی
نویسندگان
1 کارشناس ارشد بیوتکنولوژی، دانشکده کشاورزی دانشگاه بوعلی سینا، همدان
2 دانشیار گروه بیوتکنولوژی، دانشکده کشاورزی دانشگاه بوعلیسینا، همدان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Medicinal plants are valuable natural resources, so recently; attention has focused on phytochemicals as new sources of natural antioxidants. Nepeta crispa and Elaeagnus angustifolia, which were used as medicinal plants from Hamedan Province in traditional medicine screened for total phenols, flavonoids, and free radical scavenging activity. In this study, flowers and aerial parts of N. crispa and E. angustifolia were extracted using methanol and hydroalcoholic solvents after drying and then total phenolic compounds were measured using Folin-Ciocalteu. Flavonoid content was determined using aluminum chloride and antioxidant properties were measured using DPPH free radical scavenging The results showed that the total contents of phenols and flavonoids in the methanolic extracts were more than of both hydroalcoholic extracts. Inhibition of DPPH free radicals increased with increasing concentration of plant extracts. The most inhibition of free radical DPPH measured by methanol extracts of N. crispa in 0.137 mg/ml, and then in E. angustifolia in 0.142 mg/ml . Results from the study indicate that the antioxidant activities of these plants may be related with total phenols and flavonoids.
کلیدواژهها [English]
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